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14α-Hydroxylation of Androst-4-en-3, 17-dione by the Whole Cells of Cyanobacterium Nostoc piscinale

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Author(s): A. Kalbasi | M.A. Faramarzi | M.S. Hejazi | H. Jahandar | M. Amini | S.M. Jalali

Journal: Biotechnology
ISSN 1682-296X

Volume: 8;
Issue: 3;
Start page: 370;
Date: 2009;
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Keywords: Nostoc piscinale | microalgae | bioconversion | steroid | androst-4-en-3 | 17-dione

ABSTRACT
The potential of microalga Nostoc piscinale was evaluated in bioconversion of androst-4-en-3, 17-dione (I). Bio-reaction was performed in BG-11 liquid medium supplemented with 0.05% steroid substrate under continuous light photo-regime of 3000 lux at 25°C for 5 days. Three major metabolites were purified chromatographically and identified on the bases of their spectral data and physical constants as 17β-hydroxyandrost-4-en-3-one (II), 14α-hydroxyandrost-4-en-3,17-dione (III) and 14α,17β-dihydroxyandrost-4-en-3-one (IV). Bioconversion characteristics observed were 14α-hydroxylation and 17-keto reduction. Present results showed that the green biocatalyst is suitable for some specific alterations on androst-4-en-3,17-dione including 14α-hydroxylation.
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