Academic Journals Database
Disseminating quality controlled scientific knowledge

6. Studies on antineoplastic enzyme producing bacteria from soil

Author(s): K.D. Kamble | P.J. Khade

Journal: International Journal of Pharmaceutical and Biomedical Research (IJPBR)
ISSN 0976-0350

Volume: 03;
Issue: 02;
Start page: 94;
Date: 2012;
VIEW PDF   PDF DOWNLOAD PDF   Download PDF Original page

Keywords: Soil bacteria | L-asparaginase | L-asparagine | Anticancer enzymes | Assay

Due to the hazards of chemotherapeutic drugs and its painful effects L-asparaginase are emerging as safer source of anticancer enzymes. Genetic studies and enzyme purification studies has always been simpler with regards to bacteria hence bacteria are also preferred source for L-asparaginase production. Study so far reports some of the bacterial L-asparaginase is allergic and therefore there is a considerable need to find alternative bacterial L-asparaginase. We have employed a media containing L-asparagine as principle carbon source for screening of l-asparaginase producing bacteria. Twenty isolates were obtained after screening various soil samples. A qualitative assay was performed in order to select the efficient bacteria. Out of these isolates twelve efficient bacteria are further selected for physiological studies and carbon sources utilization. Optimization of pH and temperature is also studied. We have also performed a comparative assay of these isolates for L-asparaginase production. The bacterial genera belong to Paenibacillus species, B. subtilis, Aneurinibacillus species, Staphylococcus species, S. saprophyticus, Brevibacillus species and Micrococcus species.

Tango Rapperswil
Tango Rapperswil

     Affiliate Program