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Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60

Author(s): Martha Mesa-Villanueva | Magaly Rueda-Martínez | Ruby Zayas-Camacho | Diana Sicard-Suárez

Journal: Universitas Scientiarum
ISSN 0122-7483

Volume: 14;
Issue: 1;
Start page: 41;
Date: 2009;
Original page

Keywords: blood mononuclear cells | comet assay | DNA damage | gamma radiation | melatonin

Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source).
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