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Accurate Quantification of Saccharin Using Isotope Dilution Liquid Chromatography Mass Spectrometry (ID-LC/MS)

Author(s): Yunjung Lee | Byungjoo Kim | Jeongkwon Kim | Seonghee Ahn

Journal: Mass Spectrometry Letters
ISSN 2233-4203

Volume: 2;
Issue: 2;
Start page: 37;
Date: 2011;

Keywords: Saccharin | Isotope analogue-13C1-sodium saccharin | Isotope dilution mass spectrometry (ID-MS) | Repeatability

Saccharin is a commonly used artificial sweetener in foodstuffs. However, for its carcinogenic dispute, it has beenregulated by government bodies. In this study, isotope dilution mass spectrometry (ID-MS) was introduced for the accuratequantification of saccharin. To employ ID-LC/MS, we obtained its isotope analogue, 13C1−sodium saccharin, by customized synthesis.Samples were spiked with 13C1-sodium saccharin and analyzed with LC/MS in negative mode. Chromatographic conditionswere optimized for the adequate chromatographic retention and separation of saccharin with a C18 column. MS was operatedwith electrospray ionization by the selected ion monitoring (SIM) mode of [M − H]− for saccharin (m/z 182) and [M − Na]− forits isotope analogue (m/z 183). To validate the ID-LC/MS method for accurate measurement, we prepared a batch of a candidatematerial by sortifying quasi−tea−drinks with saccharin and analyzed samples gravimetrically fortified in various levels of concentration.The repeatability and reproducibility of this method was tested by analyzing the reference material. Result show thatID-LC/MS is a reliable method for the quantitative analysis of saccharin.
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