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Adaptation of Infectious Bursal Disease Virus (IBDV) on Vero Cell Line

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Author(s): M.M. Ahasan | K.M. Hossain | M.R. Islam

Journal: Journal of Biological Sciences
ISSN 1727-3048

Volume: 2;
Issue: 9;
Start page: 633;
Date: 2002;
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Keywords: Infectious bursal disease virus | vero cell line

ABSTRACT
This experiment was conducted to study the adaptation of IBDV on vero cell line. Suspected IBDV isolates were collected from the bursa of dead chicken of a particular flock. For adaptation, the anchorage-dependant monolayer of vero cell was first subcultured to form semi-confluent monolayer in eagle`s minimum essential medium (EMEM) with 5% fetal calf serum. This semi-confluent monolayer was then infected by local field IBDV isolates. The passage 1 (P1) viruses were harvested and used for the next passage. In this way, the viruses were given three serial passages on vero cell line where characteristic cytopathic effects (CPEs) were observed. During the first passage, no CPEs were found. The infectivity of IBDV on vero cells was observed by changes in the characteristics of vero cell monolayer. During the third passage, clear and consistent CPEs were observed. Vero cell monolayer was changed to form rounding cells. The P3 adapted viruses were confirmed to be IBDV by agar gel precipitation test. The P3 IBDV virus became well adapted to vero cell line.
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