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Age features in protein’s composition modifications of the neurospecifical and glial intermediate filaments in different parts of the red’s brain depend on ionization radiation actions

Author(s): Romanenko L.A.

Journal: Морфологія
ISSN 1997-9665

Volume: 2;
Issue: 2;
Start page: 61;
Date: 2008;
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Keywords: brain | neurofilaments | glial fibrillar acidic protein | ionization radiation

In this work we compare the main changes in protein composition of intermediate filaments due to ionization radiation actions on old and yang rats. The objects of our investigation were 120 rats (60 old and 60 yangs). They were divided on groups in depend on ionization radiation action duration – 1 day, 1,2 and 3 week, and the control group. The action of ionization radiation was modeled with the help of “РУМ-17” in the fate 0,00129 Kl/kg. Brains were divided on parts (cortex, cerebellum, gypocamp), homogenized, centrifugised. The quantity of glial and neuronal filaments (NF) was defined by the rocket-lineal immunoelectrophoresis; immunoblotting with specific antibodies was used for qualitative changes defining. After ionization radiation action we observed no age features in protein composition of intermediate filaments. All changes were due to duration of the ionization radiation action. Main changes in neurofilaments were associated with decrease of 210 kDa subunit. Products of degradation (40 - 48 kDa) of fibrillar glial filaments were observed on the 2 week of experiment. There were biphasic changes in glial fibrillar acidic protein in cerebellum and gypocamp of yang and old rats.
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