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Agrobacterium-Mediated Transformation of indica Rice (Oryza sativa L.), IR64 with Mungbean LEA Protein Gene for Water-Stress Tolerance

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Author(s): S. Rajesh | S. Krishnaveni | D. Sudhakar | M. Raveendran | P. Sivakumar | R. Gnanam | A. Manickam

Journal: American Journal of Plant Physiology
ISSN 1557-4539

Volume: 3;
Issue: 3;
Start page: 101;
Date: 2008;
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Keywords: Agrobacterium | indica rice | LEA | mature seed-derived calli | rice transformation | transgenic rice

ABSTRACT
Late embryogenesis abundant proteins are associated with tolerance to water-stress resulting from desiccation and cold shock. A LEA protein gene, emv2 from Vigna radiata was sense expressed into indica rice cultivar IR 64 to produce plants tolerant to limited water stress situations. Mature seed-derived embryogenic calli were transformed with Agrobacterium strain LBA 4404 harboring the binary vector pCEMV2 with mungbean emv2 gene. Active proliferating resistant calli selected in hygromycin and cefotaxime were regenerated and transferred to half-MS rooting medium. The putative transgenic rice plants expressing emv2 were analyzed for integration of the transgene through polymerase chain reaction and tested positive for hph and emv2 genes.
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