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Alpha-enolase immunohistochemical study of stem cels in limbal autograft tissue

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Author(s): Kozák I. | Trbolová Alexandra | Kolodzyeiski L. | Juhás T. | Ledecky V. | Rosocha J.

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 53;
Issue: 4;
Start page: 269;
Date: 2003;
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Keywords: alpha-enolase | immunohistochemistry | stem cells | limbal autotransplantation | chemical eye injury

ABSTRACT
The purpose was to detect the presence of stem cells in the limbus and cornea following limbal autotransplantation for chemical eye injury. Fifteen New Zealand white rabbits were used in the study. The first group (n=5) served as healthy controls, in group 2 (n=5) one eye of each rabbit was burnt by 1 N NaOH and in the last group (n=5), the same chemical injury was followed by limbal autotransplantation from the contralateral eye. The eyes were examined clinically and studied by immunohistochemistry after the enucleation. A monoclonal antibody against alpha-enolase - a biochemical marker for stem cells, was used for immunohistochemistry. The density of cells in both limbus and the cornea was calculated per mm2. The Tukey-Kramer parametric test was used for statistical evaluation. Healthy limbus and basal corneal layers showed numerous a-enolase positive cells. The injured group showed marked depletion of these cells (p
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