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Alpha B-crystallin is a new prognostic marker for laryngeal squamous cell carcinoma

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Author(s): Mao Yuan | Zhang Da-Wei | Lin Hong | Xiong Lin | Liu Ying | Li Qing-Dong | Ma Jun | Cao Qing | Chen Ren-Jie | Zhu Jin | Feng Zhen-Qing

Journal: Journal of Experimental & Clinical Cancer Research
ISSN 1756-9966

Volume: 31;
Issue: 1;
Start page: 101;
Date: 2012;
Original page

Keywords: Laryngeal carcinoma | Alpha B-crystallin gene | RT-PCR | qPCR | Immunohistochemistry | Prognosis

ABSTRACT
Abstract Background Alpha B-crystallin (αB-crystallin) has been suggested to play an important role in the development of solid tumors. However, the association between αB-crystallin expression and clinicopathological characteristics of human laryngeal carcinoma is not well defined. This study aimed to examine the expression of αB-crystallin in human laryngeal squamous cell carcinoma (LSCC) and investigate the relationship between its expression and the prognosis of LSCC. Methods Real-time polymerase chain reaction (six LSCC samples, six tumor-adjacent normal samples) and immunohistochemistry by tissue microarrays (109 LSCC samples and 28 tumor-adjacent normal samples) were performed to characterize expression of the αB-crystallin gene in LSCC. Kaplan-Meier survival and Cox regression analyses were carried out to evaluate the prognosis of LSCC. Results Real-time polymerase chain reaction and immunohistochemistry analysis showed that the expression of αB-crystallin in LSCC was significantly higher than that in tumor-adjacent normal tissues. Moreover, the expression level of αB-crystallin protein in LSCC was significantly related to alcohol consumption (P = 0.022), tumor differentiation (P = 0.007), pTNM stage (P = 0.041) and 5 years’ survival (P =0.030). COX multi-factor analysis showed that αB-crystallin (P = 0.013), as well as pTNM stage (P =0.027) and lymphatic metastasis (P = 0.015) were independent prognosis factors for LSCC. Conclusions The data suggest that αB-crystallin expression is correlated with malignant phenotypes of LSCC and it may serve as a novel prognostic factor for LSCC.

Tango Jona
Tangokurs Rapperswil-Jona

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