Academic Journals Database
Disseminating quality controlled scientific knowledge

Alteration of the Expression Levels of Rab3A Affects the Extent of Transcytosis of HRP-Labeled Marker Proteins in Rat CNS Neurons

Author(s): Yoshiki Takeuchi | Yoshiki Matsumoto | Takanori Miki | Katsuhiko Warita | Zhi-Yu Wang | Kuldip S. Bedi | Tomiko Yakura | Jun-Qian Liu

Journal: Neuroscience & Medicine
ISSN 2158-2912

Volume: 02;
Issue: 03;
Start page: 282;
Date: 2011;
Original page

Keywords: Rab3A-siRNA | WGA-HRP | Transcytosis | Synapse | Vagus Nerve | Nucleus of Solitary Tract | Vesicle Docking | Quantitative Real Time RT-PCR

It has been hypothesized that Rab3A, a small GTPase, may be closely involved in the process of dense core vesicle exocytosis in various cell types. This possibility was investigated by disrupting the expression levels of Rab3A-mRNA using a small interfering RNA of the Rab3A GTPase (Rab3A-siRNA) and examining the effect of this on transcytosis of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP). Rab3A-siRNA and WGA-HRP were injected into the right vagus nerves of adult rats which were killed 12, 24 or 48 hours later. In some animals, portions of the brain stem containing the nucleus of solitary tract (NST) were prepared for electron microscopy. In other animals, the nodose ganglion of the vagus nerve was used to determine the levels of expression of Rab3A-mRNA using RT-PCR techniques. It was found that the expression of Rab3A-mRNA was markedly depressed in animals at 12 h after the Rab3A-siRNA injection. In the NST, there was an accumulation of HRP-reaction product (RP), recognized as electron dense lysosomal-like structures, in both axons and terminals in the NST 12 h after injection. Some HRP-RP was found in membrane bound vesicles in close proximity to cell membranes and appeared to be in the process of transcytosis. This neuronal transcytosis of HRP-RP appeared to occur at random locations over the axodendritic membranes. These findings indicate that inhibiting the expression of Rab3A-mRNA using Rab3A-siRNA can modulate the level of transcytosis of proteins across neuronal membranes confirming the potentially important role of this GTPase in the process of transcytosis.

Tango Rapperswil
Tango Rapperswil

     Affiliate Program