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Aluminum hydroxide associated to Schistosoma mansoni 22.6 kDa protein abrogates partial protection against experimental infection but not alter interleukin-10 production

Author(s): Lucila GG Pacífico | Cristina T Fonseca | Michele M Barsante | Luciana S Cardoso | Maria Ilma Araújo | Sérgio C Oliveira

Journal: Memórias do Instituto Oswaldo Cruz.
ISSN 0074-0276

Volume: 101;
Start page: 365;
Date: 2006;
Original page

Keywords: Schistosoma mansoni | Sm22.6 | recombinant vaccine | aluminum hydroxide | adjuvant | interleukin-10

The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91%, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6 induced a partial protection of 34.5% when used together with Freund's adjuvant and produced a Th0 type of immune response with interferon-g and interleukin-4. In this work, mice were immunized with Sm22.6 alone or with aluminum hydroxide adjuvant and high levels of IgG, IgG1, and IgG2a were measured. Unfortunately, no protection was detected. Since IL-10 is a modulating cytokine in schistosomiasis, we also observed a high level of this molecule in splenocytes of vaccinated mice. In conclusion, we did not observe the adjuvant effect of aluminum hydroxide associated with rSm22.6 in protective immunity.

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Tango Rapperswil

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