Academic Journals Database
Disseminating quality controlled scientific knowledge

Aluminum hydroxide associated to Schistosoma mansoni 22.6 kDa protein abrogates partial protection against experimental infection but not alter interleukin-10 production

ADD TO MY LIST
 
Author(s): Lucila GG Pacífico | Cristina T Fonseca | Michele M Barsante | Luciana S Cardoso | Maria Ilma Araújo | Sérgio C Oliveira

Journal: Memórias do Instituto Oswaldo Cruz.
ISSN 0074-0276

Volume: 101;
Start page: 365;
Date: 2006;
Original page

Keywords: Schistosoma mansoni | Sm22.6 | recombinant vaccine | aluminum hydroxide | adjuvant | interleukin-10

ABSTRACT
The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91%, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6 induced a partial protection of 34.5% when used together with Freund's adjuvant and produced a Th0 type of immune response with interferon-g and interleukin-4. In this work, mice were immunized with Sm22.6 alone or with aluminum hydroxide adjuvant and high levels of IgG, IgG1, and IgG2a were measured. Unfortunately, no protection was detected. Since IL-10 is a modulating cytokine in schistosomiasis, we also observed a high level of this molecule in splenocytes of vaccinated mice. In conclusion, we did not observe the adjuvant effect of aluminum hydroxide associated with rSm22.6 in protective immunity.

Tango Rapperswil
Tango Rapperswil

     Affiliate Program