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Amelioration of Chilling Injuries in Mung Bean (Vigna radiata L.) Seedlings by Paclobutrazol, Abscisic Acid and Hydrogen Peroxide

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Author(s): Amal A.H. Saleh

Journal: American Journal of Plant Physiology
ISSN 1557-4539

Volume: 2;
Issue: 6;
Start page: 318;
Date: 2007;
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Keywords: Chilling stress | paclobutrazol | abscisic acid | H2O2 | antioxidant enzymes | mung bean (Vigna radiata L.) plant

ABSTRACT
The present study was carried out to study the effect of chilling stress on some metabolic products and antioxidative system in mung bean (Vigna radiata L.) plant and try to alleviate chilling injuries by using paclobutrazol, abscisic acid and H2O2. Twenty-day-old seedlings were randomly separated into equal three groups, the first group was left in green house at 35°C, the second one was chilled at 5°C for 5 or 10 h and the last one was treated with 0.0, 25 and 50 mg L-1 paclobutrazol, 0.5 and 1 mM abscisic acid or 0.0, 0.1 and 0.5 mM H2O2 before transforming to 5°C. The results showed that, chilling stress induced significant increased lipid peroxidation, membrane leakage and hydrogen peroxide level, while activities of catalase, peroxidase and ascorbate peroxidase were decreased. In addition, total chlorophyll, total carbohydrates, protein content and proline level decreased following exposure to 5°C. Paclobutrazol, abscisic acid and H2O2 treatments ameliorated the chilling injuries by lowering lipid peroxidation, membrane leakage and H2O2 level and increasing in total chlorophyll, carbohydrates, protein content, proline level and antioxidant enzymes activities. Based on these results, it was presumed that the stress protection caused by paclobutrazol, abscisic acid and H2O2 probably contributes to some extent to the enhanced activity of the free-radical scavenging systems.
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