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Análisis de dos poblaciones de gallinas criollas (Gallus domesticus) utilizando RAPD´s como marcadores moleculares

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Author(s): Guadalupe Zavala Páramo | Horacio Cano Camacho | Irma Morelia Soto Huipe | Joel E. López Meza

Journal: Técnica Pecuaria en México
ISSN 0040-1889

Volume: 40;
Issue: 3;
Start page: 275;
Date: 2002;
Original page

Keywords: Native chickens | Egg production | Molecular markers | RAPD¿s

ABSTRACT
México has a great variety of native poultry but knowledge about its diversity is minimal. In this study, twenty individuals belonging to two populations of native hens (Gallus domesticus) were analyzed. They were chosen by egg production, through polymorphism identification generated by DNA random amplification (RAPD¿s). Amplification generated products show different sizes between 0.2 to 1.1 kb. Polymorphism was detected between populations and inside populations, being the low egg production hen population the one which showed the lowest variability. Both populations show conserved products at 0.2 kb, 0.4 kb, 0.5 kb and 0.6 kb. The 1.1 kb fragment present in the lower egg production hen population did not show up in the other population, thus indicating that it could be a candidate as a genetic marker for characterization of hens with low egg production; or that the absence of this fragment could indicate hens with good egg production. The RAPD technique is a useful tool for identifying polymorphisms in a population and preliminary results allow to establish molecular variability between hen populations.
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