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Analysing the dhaT gene in Colombian Clostridium sp. (Clostridia) 1,3-propanediol-producing strains

Author(s): Diana Milena Quilaguy-Ayure | José David Montoya-Solano | Zulma Rocío Súarez-Moreno | José Mauricio Bernal-Morales | Dolly Montoya-Castaño

Journal: Universitas Scientiarum
ISSN 0122-7483

Volume: 15;
Issue: 1;
Start page: 17;
Date: 2010;
Original page

Keywords: 1 | 3-propanediol | 1 | 3-propanediol dehydrogenase | dhaT gene | 1 | 3-propanediol operon

To analyze the dhaT gene, one of the genes responsible for the 1,3-propanediol (1,3-PD) production, in two native Clostridiumstrains. Materials and methods: The dhaT gene was amplified by Polimerase Chain Reaction with specific primers designed fromClostridium butyricum VPI1718 operon. Bioinformatics tools like BLASTN, ORF finder, BLASTP and ClustalW were used to determinethe identity of the sequence and to assign a function. Results: DNA amplification products were obtained from Colombian Clostridium sp.native strains (IBUN 13A and IBUN 158B) and the Clostridium butyricum DSM 2478 strain, which were sequenced. According to thebioinformatics analysis of the above sequences, a high degree of similarity was found with the dhaT gene of different bacterial species. Thehighest percentage of identity was obtained with the Clostridium butyricum VPI 1718 strain. Conclusion: knowledge of the physicalstructure of the 1,3-PD operon in native strains opens the way for developing genetic and metabolic engineering strategies for improvingprocesses productivity.
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