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The analysis of alcoholic extracts of hypericum species by UV/VIS spectrophotometry

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Author(s): DanielaGÎTEA | Monica ŞIPOŞ | TĂMAŞ MIRCEA | Bianca PAŞCA

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVII;
Issue: 1;
Start page: 111;
Date: 2010;
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Keywords: Hypericum | UV/VIS spectrophotometry | flavones | hypericins

ABSTRACT
In this report, the UV/VIS spectrophotometric method was employed in order to identify and dose the active principles contained in the alcoholic extracts of Hypericum perforatum L., Hypericum maculatum Crantz, with two subspecies, Hypericum tetrapterum Fries and Hypericum hirsutum L. The most important active principles are hypericin and flavones. For the identification of flavones an officinal method in FR X was used, whereas for the hypericin - an officinal method in the European Pharmacopoeia. The obtained results led to the conclusion that H. maculatum has the highest content of total hypericins (0.496g%) as well as flavonoids (6.418g%). In the European Pharmacopoeia the St. John’s wort monograph specifies the exact content of hypericin of the vegetable product (determined through the spectophotometric method), of 0.08g% total hypericin. Given this fact, all the studied Hypericum species fit within the limits imposed by the E. Ph. There is no stipulation in FR X regarding the limits of hypericin concentrations or of other active principles that can be found in Hyperici herba extracts.
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