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THE ANALYSIS OF CATALASE AND PEROOXIDASE ACTIVITY IN SAPROPHYTIC FUNGUS RHIZOPUS NIGRICANS GROWN ON MEDIUM WHITH DIFERENT CONCENTRATION OF GRINDED CORN CARYOPSIS

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Author(s): Tamara Barbaneagra | Alexandru Manoliu | Mihaela Cristica | Elena Ciornea | Elena Tutu

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 12;
Issue: 3;
Date: 2010;
Original page

ABSTRACT
The purpose of this study was to assay catalase and peroxidase activity in the saprophytic fungus Rhizopus nigricans, grown on mediums containing grinded corn caryopsis, which, in our experiments have replaced carbon source – sucrose in composition of liquid culture medium Czapeck Dox, resulting in the final three experimental variants: V1 = 20 g/l, V2 = 30 g/l, V3 = 40 g/l, while the control variant composition remained unchanged. Measurements were made at three time intervals: 5 days and 10 days and 15 days after inoculation, using fungus mycelium and culture liquid. Determination of catalase activity was performed using Sinha method (Artenie Vl., et al., 2008), and determination of peroxidase was carried out on the basis of ortho-dianisidine method (Cojocaru D.C., 2009). The results showed significant deferens in dynamic of enzyme activity depending on the concentration of carbon source introduced into the medium and age of the fungus.

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