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Analysis of the Sequence of ITS1-5.8S-ITS2 Regions of the Three Species of Fructus Evodiae in Guizhou Province of China and Identification of Main Ingredients of Their Medicinal Chemistry

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Author(s): Liu-Zhongyu | Liu-Yang | Liu-Shouhan | Ding-Xianping | Yang-Yi, et al

Journal: Journal of Computer Science & Systems Biology
ISSN 0974-7230

Volume: 02;
Issue: 03;
Start page: 200;
Date: 2009;
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Keywords: Fructus Evodiae | ITS | Phylogenetic Analysis | HPLC | Identification

ABSTRACT
A total of fourteen specimens of three Fructus Evodiae species were collected from Zunyi and Tongren of Guizhou Province in China. The internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear ribosomal DNA (nrDNA) of the fourteen species were amplified and sequenced and another two sequences of Evodia rutaecarpa (JUSS.) BENTH species were obtained from Hunan Academy of Traditional Chinese Medicine, Changsha, Hunan Province of China. In addition to, the main ingredients of their medicinal chemistry of all specimens were identified by high performance liquid chromatography (HPLC). The three different morphological Fructus Evodiae species could not be distinctly classified by phylogenetic analysis of ITS combining the main ingredients of medicinal chemistry. This conclusion is inconsistent with early research results.
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