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Analysis of SNPs in the KIT gene of cattle with different coat colour patterns and perspectives to use these markers for breed traceability and authentication of beef and dairy products

Author(s): Luca Fontanesi | Emilio Scotti | Vincenzo Russo

Journal: Italian Journal of Animal Science
ISSN 1594-4077

Volume: 9;
Issue: 2;
Start page: e42;
Date: 2010;
Original page

Keywords: Breeds | Cattle | Coat colour | KIT | SNPs.

The identification of the breed of origin of farm animals has recently assumed particular relevance as increasing interests in marketing mono-breed labelled lines of beef and dairy products have created the need to protect them from frauds. In order to develop DNA based breed traceability and authentication protocols, the first step is the identification of breed specific markers with high discriminatory power among breeds. We analysed two single nucleotide polymorphisms identified in exon 2 (g.72779776C>T) and exon 3 (g.72783182A>G) of the KIT gene (a candidate gene for the spotting locus) in seven cattle breeds with different coat colour patterns (Italian Holstein-Friesian, no. = 61; Italian Brown, no. = 60; Italian Simmental, no. = 78; Jersey, no. = 60; Rendena, no. = 51; Reggiana, no. = 128; and Modenese, no. = 52). The two alleles of both SNPs were detected in all analysed breeds making their use unsuitable in breed traceabilty with a deterministic approach. Italian Simmental was almost fixed for the most common alleles (g.72779776C and g.72783182A). Haplotype analysis showed that spotted breeds (Italian Holstein-Friesian and Italian Simmental) had only two haplotypes with one of them ([C:A]) with high frequency (~90% and ~99%, respectively). Analysis of molecular variance (AMOVA) averaged over the two loci indicated that genetic variation between spotted and non-spotted groups of breeds amounted to 25.3% (P

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