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Anatomical structure of african violet (Saintpaulia ionantha L.) vitro- and exvitroplantlets

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Author(s): Adriana PETRUŞ-VANCEA | Angela Monica ŞIPOŞ

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVII;
Issue: 2;
Start page: 219;
Date: 2010;
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Keywords: vitroculture | acclimatization | Saintpaulia | anatomical structure

ABSTRACT
In this article we study the histo-anatomical structure of vegetative organs of African violet (Saintpaulia ionantha L.) vitro- and exvitroplantlets in comparison with similar aspects at the same organs of the greenhouse plants (control lot). The phytoinoculs vitroculture period was 120 days, the ex vitro acclimatization for the exvitroplantlets needed 30 days, and the greenhouse cultivar was 2 years old. Finally, we found that only rootlets of the vitroplantlets had a primary structure because at stemlets level has been identified the cambium presence still the vitroculture period. The cortical parenchyma cells at vitro- and exvitroplantlets was larger and less compact in comparison with those of control lot. Also, in the vitroplantlet rootles and stemlets the report cortex:central cylinder was much higher and vascular bundle was very poorly represented that at exvitroplantlets, but especially in comparison with these aspects in the plants grown in natural conditions. The spongy parenchyma at leaflets from in vitro culture was composed of fewer cell layers which was larger and less compact in comparison with those of exvitroplantlet leaf homologous layers and with the same layers from the leaf of greenhouse plants. At in vitro leaflets the peryphloemic protective mechanical tissue was at an early forming stage. However, we consider these differences as being due to the plants normal ontogenetic development.
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