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Antagonist capacity of Newly Isolated Strains of Pseudomonas Fluorescens against Three Important Phytopathogenic Bacteria

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Author(s): Reynaldo D.L. Cruz-Quiroz | Catalina Chavez | Marcela Hernandez | Raul Rodriguez | Daniel Hernandez | Cristobal N. Aguilar

Journal: American Journal of Agricultural and Biological Science
ISSN 1557-4989

Volume: 6;
Issue: 2;
Start page: 267;
Date: 2011;
Original page

Keywords: Pseudonomas fluorescens | ellular nature | Mexican agriculture | Phytopatogen bacteria | foliar spots | Free Cell crude extracts (FC) | fluorescens strains | polypeptone | microorganisms | economical losses

ABSTRACT
Problem statement: Phytopatogenic bacteria cause several damages to plants with important economical consequences. They provoke losses of product quality affecting all commercial chain of crops, for this reason, their control is a priority. Approach: We evaluated antagonist capacity of newly isolated Pseudonomas fluorescens strains against three important phytopatogenic bacteria (Clavibacter michiganensis, Xanthomonas axonopodis and Erwinia carotovora). Soils from commercial cropping of Capsicum annum L of several Mexican regions were used to isolate P. fluorescens strains. Results: Isolates producing flourescein were purified on King B agar and biochemically identified. Crude extracts with and without cells were produced in King B broths and their antagonist capacities were evaluated by the plate diffusion procedure on nutritive agar. Conclusion: Obtained results demonstrated that cell free extracts exhibited a limited antagonist capacity in comparison of those extracts with cells, which showed an excellent capacity to inhibit the growth of C. michiganensis, X. axonopodis and E. carotovora, demonstrating the intracellular nature of the bioactive metabolites associated to bacterial growth inhibition.
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Tango Rapperswil
Tango Rapperswil