Author(s): Aseer Manilal | B. Sabarathnam; G.S. Kiran; S. Sujith; C. Shakir and J. Selvin
Journal: Asian Journal of Medical Sciences
ISSN 2040-8765
Volume: 2;
Issue: 4;
Start page: 195;
Date: 2010;
Original page
Keywords: Antimicrobial compound | Aspergillus clavatus | marine fungi | marine sponge | multidrug resistant pathogens
ABSTRACT
The development of resistance to multiple drugs is a major problem in the treatment of these infectious diseases. Multidrug Resistant Staphylococcus aureus (MRSA) and Candida sp, the major infectious agents have been recently reported in quite a large number of studies. With more intensive studies for natural therapies, marine-derived products have been a promising source for the discovery of novel bioactive compounds. A total of 45 marine fungi were isolated from the two sponges F. cavernosa and D. nigra were screened for antimicrobial activity against pathogenic bacteria and fungi. The novel basal media formulated in the present study resulted in increased frequency of fungal isolates when compared to all other media used in the present study. The cell free supernatant of fungi exhibiting the broad spectrum of activity was subjected to chemical analysis using different chromatographic systems including TLC, Column and GC-MS. Of the 15 fungal strains, 20% (3 strains) showed potential antagonistic activity against a panel of clinical pathogens used in the present study. Based on the antimicrobial activity of the isolates, Aspergillus clavatus MFD15 was recorded as potent producer displaying 100% activity against the tested pathogenic organisms. The TLC of the crude ethyl acetate extract produced 3 spots with Rf values of 0.20, 0.79 and 0.95, respectively. The active TLC fraction was purified in column chromatography which yielded 50 fractions. The active column fractions were combined and analyzed with FT-IR, UV-Vis and GC-MS. The chemical analysis of the active compound envisaged the active compound to be a triazole, 1H-1,2,4 Triazole 3- carboxaldehyde 5- methyl. The triazolic compound was bacteriostic for S. aureus and bactericidal for E. coli. The triazole treated fabric showed 50% reduction in the growth of E. coli, S. aureus, and S. epidermidis. Thus the purified compound can find a place in the database for the development of fabrics with antimicrobial properties. This is the first report that envisaged the production of triazole antimicrobial compound from sponge associated marine fungi from the Indian coast.
Journal: Asian Journal of Medical Sciences
ISSN 2040-8765
Volume: 2;
Issue: 4;
Start page: 195;
Date: 2010;
Original page
Keywords: Antimicrobial compound | Aspergillus clavatus | marine fungi | marine sponge | multidrug resistant pathogens
ABSTRACT
The development of resistance to multiple drugs is a major problem in the treatment of these infectious diseases. Multidrug Resistant Staphylococcus aureus (MRSA) and Candida sp, the major infectious agents have been recently reported in quite a large number of studies. With more intensive studies for natural therapies, marine-derived products have been a promising source for the discovery of novel bioactive compounds. A total of 45 marine fungi were isolated from the two sponges F. cavernosa and D. nigra were screened for antimicrobial activity against pathogenic bacteria and fungi. The novel basal media formulated in the present study resulted in increased frequency of fungal isolates when compared to all other media used in the present study. The cell free supernatant of fungi exhibiting the broad spectrum of activity was subjected to chemical analysis using different chromatographic systems including TLC, Column and GC-MS. Of the 15 fungal strains, 20% (3 strains) showed potential antagonistic activity against a panel of clinical pathogens used in the present study. Based on the antimicrobial activity of the isolates, Aspergillus clavatus MFD15 was recorded as potent producer displaying 100% activity against the tested pathogenic organisms. The TLC of the crude ethyl acetate extract produced 3 spots with Rf values of 0.20, 0.79 and 0.95, respectively. The active TLC fraction was purified in column chromatography which yielded 50 fractions. The active column fractions were combined and analyzed with FT-IR, UV-Vis and GC-MS. The chemical analysis of the active compound envisaged the active compound to be a triazole, 1H-1,2,4 Triazole 3- carboxaldehyde 5- methyl. The triazolic compound was bacteriostic for S. aureus and bactericidal for E. coli. The triazole treated fabric showed 50% reduction in the growth of E. coli, S. aureus, and S. epidermidis. Thus the purified compound can find a place in the database for the development of fabrics with antimicrobial properties. This is the first report that envisaged the production of triazole antimicrobial compound from sponge associated marine fungi from the Indian coast.
