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Anti-bacterial Evaluation and Minimum Inhibitory Concentration Analysis of Oxalis corniculata and Ocimum santum against Bacterial Pathogens

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Author(s): Md. Shahedur Rahman | Md. Mahboob Hossain Khan | Md. Abu Hena Mostofa Jamal

Journal: Biotechnology
ISSN 1682-296X

Volume: 9;
Issue: 4;
Start page: 533;
Date: 2010;
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Keywords: Oxalis corniculata | antibacterial activity | Medicinal plants | Ocimum santum | MIC

ABSTRACT
In this present study, it is tried to find out the antimicrobial effect and Minimum Inhibitory Concentration (MIC) of Oxalis corniculata leaf and Ocimum sanctum leaf extract against clinical isolates from urine, stool and sputum and their successive comparison with commercially, available antibiotic discs. Powdered leaves were prepared and used for extraction with various solvents, viz., methanol, ethanol and chloroform in case of Oxalis corniculata and ethanol, methanol, n-hexane and petroleum spirit in case of Ocimum santum. All the solvent extracts were evaporated to dryness in air. Using the disc diffusion method, the bacterial growth were inhibited. Among the solvent extracts tested, methanol extract of Oxalis corniculata leaf showed higher antibacterial activity compared to Erythromycin and Nalidixic acid against Staphylococci sp. whereas the methanol extract of Ocimum sanctum leaf showed higher antibacterial activity compared to CIP-5 against Staphylococcus aureus. The best MIC values were recorded to be 256 μg mL-1 against Salmonella typhi for ethanol extract of Oxalis corniculata leaf and 128 μg mL-1 against Staphylococcus aureus for the methanol extract of Ocimum sanctum leaf.

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