Author(s): Balbir Singha*, Vandna Kalsib, Anupam Sharmac and M. P. S. Ishara
Journal: Journal of Pharmacy Research
ISSN 0974-6943
Volume: 4;
Issue: 9;
Start page: 2904;
Date: 2011;
Original page
Keywords: Ocimum sanctum | antianxiety activity | callus cultures
ABSTRACT
Callus cultures from stem of Ocimum sanctum were induced and maintained on MS-basal medium with some modifications and supplemented with NAA and Kn(1ppm each). The petroleum ether (PE), chloroform (CE), ethanol (EE) and water extractives (WE) of stem, leaf and stem calli of O. sanctum were tested forantianxiety activity against standard drug, diazepam using elevated plus maze apparatus and albino mice. The ethanol extractive of callus tissue (6-month-old)exhibited maximum significant antianxiety activity out of all extractives studied followed by ethanol extractive of stem (8-month-old) of O. sanctum. Chemicallythe extractives of stem and leaf showed the presence of saponins, sterols, triterpenoids, carbohydrates, tannins and proteins in stem, leaf and stem cultures. Whileflavonoids were detected in stem and Leaf only. The results indicate that both the original plant and callus developed from stem can be considered as potentialcandidate for bioactivity guided isolation of natural antianxiety agents.
Journal: Journal of Pharmacy Research
ISSN 0974-6943
Volume: 4;
Issue: 9;
Start page: 2904;
Date: 2011;
Original page
Keywords: Ocimum sanctum | antianxiety activity | callus cultures
ABSTRACT
Callus cultures from stem of Ocimum sanctum were induced and maintained on MS-basal medium with some modifications and supplemented with NAA and Kn(1ppm each). The petroleum ether (PE), chloroform (CE), ethanol (EE) and water extractives (WE) of stem, leaf and stem calli of O. sanctum were tested forantianxiety activity against standard drug, diazepam using elevated plus maze apparatus and albino mice. The ethanol extractive of callus tissue (6-month-old)exhibited maximum significant antianxiety activity out of all extractives studied followed by ethanol extractive of stem (8-month-old) of O. sanctum. Chemicallythe extractives of stem and leaf showed the presence of saponins, sterols, triterpenoids, carbohydrates, tannins and proteins in stem, leaf and stem cultures. Whileflavonoids were detected in stem and Leaf only. The results indicate that both the original plant and callus developed from stem can be considered as potentialcandidate for bioactivity guided isolation of natural antianxiety agents.
