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ANTIBIOTIC SUSCEPTIBILITY OF BIODEGRADING BACTERIAL ISOLATES FROM DAIRY EFFLUENT

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Author(s): Anupama Bhardwaj and Sonia Chaman*

Journal: International Journal of Bioassays
ISSN 2278-778X

Volume: 2;
Issue: 7;
Start page: 956;
Date: 2013;

Keywords: Antibiotic Disk Diffusion | Antibiotic Susceptibility | Biodegradation | Dairy Effluent | COD | BOD.

ABSTRACT
The present study investigates the prevalence of antibiotic resistance among bacterial isolates from dairy effluent sample of two different seasons from Verka Milk Plant, Mohali. Twenty bacteria were isolated by spread plating and streaking method. Variable amount of reduction in BOD and COD was observed in dairy effluentafter the treatment with free and immobilized seven bacterial strains i.e. A3, A8, A11, A13, A19, A21 and A23. Bacterial isolates with degrading efficiency were identified on the basis of standard cultural, morphological and biochemical characteristics. Maximum biodegradation was shown by A23. To get ensure about the bacteria used for biodegradation is harmless to the environment  these bacteria were evaluated for their resistance and susceptible pattern against ten commonly prescribed clinically significant antibiotics viz, Tetracycline, Cefotaxime, Amikacin, Cefixime, Ampicillin, Penicillin, Gentamicin, Cloxacillin, Erythromycin and Choramphenicol by using antibiotic disc diffusion method. All the strains showed susceptibility against Chloramphenicol, Tetracycline and Cefotaxime. All bacterial isolates has shown resistance against Cloxacillin. The analysis of antibiotic resistance frequencies has shown an incidence of 57.14% strains resistant to four or more than four different antibiotics. The high prevalence of antibiotic-resistant bacteria harboring diverse resistance trait could represent a potential health risk. The study of antibiotic resistance helps to predict future emergence and guide the development of strategies to counteract this resistance before their application in biodegradation process. The present study investigated the prevalence of antibiotic resistance among biodegrading bacterial isolates from dairy effluent and determined their resistance patterns.
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