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ANTICANCER ACTIVITY OF PONGAMIA GLABRA V. SEED OIL EXTRACT AGAINST SELECTED HUMAN CANCER CELL LINES

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Author(s): Chinnasamy Arulvasu | Subramanian Vasantha suppriya | Gajendran Babu

Journal: International Research Journal of Pharmacy
ISSN 2230-8407

Volume: 3;
Issue: 8;
Start page: 131;
Date: 2012;
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Keywords: Pongamia glabra | MCF-7 | HeLa | Trypan blue | MTT | DNA fragmentation.

ABSTRACT
Screening of the seed oil extract from Pongamia glabra V. (Fabaceae) has been carried out for antiproliferative activity of cancer cells. The seed oil was extracted with methanol and then persuasive activity was tested on human cancer cell lines MCF-7 and HeLa. The cell growth inhibitory effects of seed oil extract was observed. The cell viability was assessed using trypan blue dye exclusion method and 3-(4, 5- Dimethyl thiazol-2yl)-2, 5-dimethyltetrazolium bromide (MTT) assay. The IC50 value of the methanolic seed oil extract against MCF-7 and HeLa was found to be 6 mg/ml and 6 mg/ml respectively after 48 hours of incubation. The P.glabra seed oil extract increased the proportion of DNA fragmentation in MCF-7 and HeLa cancer cell lines. Moreover, the inhibitory effect is correlated with DNA fragmentation. These results suggest that the P.glabra seed oil extract has an inhibitory effect on human cancer cell lines MCF-7 and HeLa.
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