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Antifungal Activity of Extracts of Rosmarinus officinalis and Thymus vulgaris against Aspergillus flavus and A. ochraceus

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Author(s): S. Centeno | M.A. Calvo | C. Adelantado | S. Figueroa

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 13;
Issue: 9;
Start page: 452;
Date: 2010;
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Keywords: Aspergillus sp. | natural products | Thymus vulgaris | Rosmarinus officinalis | Biocontrol

ABSTRACT
The antifungal activity of ethanolic extracts of Rosmarinus officinalis and Thymus vulgaris were tested against strains of Aspergillus flavus and A. ochraceus, since these two species are common contaminants of cereals and grains and are able to produce and accumulate mycotoxins. The methodology used is based on measuring the inhibition halos produced by discs impregnated with the extracts and establishing their Minimum Inhibitory Concentration (MIC) as well as the Minimum Fungicide Concentration (MFC). The results obtained suggest that the assayed extracts affect the proper development of A. flavus and A. ochraceus; leading to a lower MIC (1200 ppm) and MFC (2400 ppm) for T. vulgaris extract against A. ochraceus than against A. flavus. The results show, that the extracts of Rosmarinus officinalis and Thymus vulgaris used at low concentrations could have significant potential for the biological control of fungi in foodstuffs.
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