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Antimicrobial and wound healing activities of leaves of Alternanthera sessilis Linn

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Author(s): Jalalpure Sunil | Agrawal Nitin | Patil M | Chimkode R | Tripathi Ashish

Journal: International Journal of Green Pharmacy
ISSN 0973-8258

Volume: 2;
Issue: 3;
Start page: 141;
Date: 2008;
Original page

Keywords: Alternanthera sessilis | antimicrobial | chloroform extract | wound healing

ABSTRACT
The leaves of Alternanthera sessilis (Linn.) R.Br.ex DC (Family Amaranthaceae) were exhaustively extracted by soxhlet apparatus with different solvents like, petroleum ether (40-60°C), chloroform, acetone, methanol and distilled water in ascending order of the polarity. All the five extracts were subjected to antimicrobial screening by using the cup plate and turbidimetric methods. The chloroform extract among all five extracts showed maximum zone of inhibition and significant MIC values in above two methods respectively. Hence chloroform extract was taken for the screening of wound healing activity and models adopted for wound healing activity were, incision, excision and granuloma studies. The chloroform extract of leaves of Alternanthera sessilis at a dose of 200 µg/mL (orally) in all models showed significant results. Contraction of wound area (P < 0.005) at 16 th day, time for complete epithelization in 16 days (P < 0.0001), and scar area after complete epithelization was found to be 33.2 ± 0.0730 (P < 0.0008) in excision wound model, wound breaking strength 388 ± 5.85 grams (P < 0.0001) in incision wound model, granuloma dry weight 47.7 ± 2.29, granuloma breaking strength 247 ± 10.2 (P < 0.022) in granuloma studies. All the results were significant for different parameters in wound healing activity when compared with control group. Presence of sterols in chloroform extract was also confirmed by preliminary phytochemical investigation, TLC and HPTLC methods.
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