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Aphid Transmission of Two Important Potato Viruses, PVY and PLRV by Myzus persicae (Sulz.) and Aphis gossypii (Glov.) in Hatay Province of Turkey

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Author(s): Erdal Sertkaya | Gulsen Sertkaya

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 8;
Issue: 9;
Start page: 1242;
Date: 2005;
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Keywords: Potato | potato viruses | aphid vectors | Myzus persicae | Aphis gossypii

ABSTRACT
Transmission assays by using aphid vectors were established to determine the effects of two aphid species (Myzus persicae Sulz. and Aphis gossypii Glov. on four solanacaeous test plant species (Capsicum annum L., Lycopersicon esculentum L., Physalis floridana Rydb. and Solanum tuberosum L.). Transmission rates of PVY and PLRV from potato to other test plants were investigated by DAS-ELISA assays. All experiments were carried out at 26:22±2°C (day:night) under a 16:8 h (light:dark) photoperiod conditions. Late 4th instar or early adult M. persicae and A. gossypii apterae were used in the transmission experiments. The aphids were not subjected to preacquisition starving in the assays. Each aphid species were reared on PVY-infected source potato plants for 5 h as Acquisition Access Period (AAP). Then, aphid group including 10 individuals were taken from donor plants were transplanted on to healthy receptor test plants for 15 h as Inoculation Period (IP). For PLRV transmissions, two aphid species reared on source potato plants for 24 h as AAP were transferred on to each healthy test plants as a group including 10 individuals for 24 h as IP. After IP, the aphids were removed from plants mechanically. PVY and PLRV were transmitted in higher rates by M. persicae. PVY and PLRV were more efficiently transmitted from potato to pepper seedlings among the other inoculated solanaceous test plants by both aphid species. The lowest transmission was occurred in tomato (2/15) and P. floridana (3/15) by using A. gossypii. Seed transmission of viruses were not determined by DAS-ELISA in seedlings re-obtained from experimentally infected test plants.
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