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The Apoptotic Effect of the Methanol Extract of <i>Polygonum cuspidatum</i> through Up-Regulation Death Receptor 5 and CHOP in HSC-2 Human Oral Cancer Cells

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Author(s): Jae-Gyu Jeon | Nam-Pyo Cho | Sung-Dae Cho | Hyun-Ju Yu | Ji-Ae Shin | Eun-Sun Choi

Journal: Journal of Biophysical Chemistry
ISSN 2153-036X

Volume: 03;
Issue: 01;
Start page: 1;
Date: 2012;
Original page

Keywords: &lt | i&gt | Polygonum cuspidatum&lt | /i&gt | Endoplasmic Reticulum Stress | C/EBP Homologous Protein/Growth Arrest and the DNA Damage-Inducible Gene 153 (CHOP) | Death Receptor 5 (DR5) | Apoptosis | Human Oral Cancer Cell

ABSTRACT
Polygonum cuspidatum is used as a traditional medicinal herb for the therapy of various diseases including several types of cancers. In the present study, we focused on addressing the anti-cancer activity and molecular mechanism of methanol extract of Polygonum cuspidatum (MEPC) in HSC-2 human oral cancer cells. The effect of MEPC on oral cancer cells was estimated by 3-(4,5-dimethylthiazol-20yl)-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium (MTS) assay, 4’-6-diamidino-2-phenylindole (DAPI) staining and Western blot analysis. MEPC inhibited the cell viability and induced apoptosis through the induction of death receptor (DR) 5. MEPC also increased the expression of C/EBP homologous protein/growth arrest and the DNA damage-inducible gene 153 (CHOP), a transcription factor induced by ER stress. Thus, we concluded that the induction of CHOP leading to DR5 up-regulation is required for the anti-cancer activity of MEPC in HSC-2 cells and MEPC may be a promising drug candidate for oral cancer.
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