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Apple shoot multiplication and plantlets reaction to in vitro culture

Author(s): Anca BUTIUC-KEUL | Adela HALMAGYI | Valentina ISAC | Cornelia CRĂCIUNAŞ | Rahela CARPA

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XVII;
Issue: 1;
Start page: 70;
Date: 2010;
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Keywords: Malus domestica | micropropagation | peroxidase | esterase | superoxid-dimutase

The present work aimed to evaluate the expression of several enzymatic systems in apple (Malus domestica Borkh., cvs. Florina, Romus3, Romus4, Colmar, Rebra, Goldrush, Idared) plants grown in vitro in comparison with the in vivo donor plants. In vitro culture was established on Murashige and Skoog (1962) basal medium supplemented with Lee and Fossard (1977) (LF) vitamins, 2 mg l-1 N6-benzyladenine, 0.01 mg l-1 N6-naphtyl-acetic acid, 30 g l-1 dextrose and 7 g l-1 agar. The highest shoot proliferation was obtained for all cultivars on medium supplemented with 1.0 mg/l N6-benzyladenine. Our study shows that in vivo plants have a distinct pattern of izoesterases in comparison with in vitro plantlets. Several izoesterases characteristic for in vitro or in vivo plants were identified. Izoperoxidases are inducible with culture conditions, physiological condition and developmental stage. The pattern of superoxid-dismutases is less variable with the culture conditions which demonstrate that in vitro culture does not occur oxidative stress. According to the pattern of peroxidases, estarases and superoxid-dismutases, there are not significant differences between in vivo and in vitro plants. Valuable apple cultivars could be preserved short or medium term by in vitro culture without genetically changes.
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