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Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows

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Author(s): Šamanc H. | Kirovski Danijela | Stojić V. | Stojanović Dragica | Vujanac I. | Prodanović R. | Bojković-Kovačević Slavica

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 61;
Issue: 5-6;
Start page: 543;
Date: 2011;
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Keywords: fatty liver | Holstein cows | metabolic profile test

ABSTRACT
The aim of this study was to examine the possibility of using results of metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. Forty dry cows, 5 to 7 days before calving, were chosen from the commercial dairy herd and included in the study. Four blood samples were taken by jugular venipuncture from each animal: 5 to 7 days before expected calving (dry period), as well as on day 12, 30 and 60 of lactation. Concentrations of glucose, β-hydroxybutyrate (BHBA), total protein, albumin, urea, total bilirubin, calcium and phosphorus concentrations were measured in all blood samples. On day 12 after calving, liver percutaneous biopsies were obtained using a biopsy instrument. Liver tissue lipid content was determined by pathohistological determination. Cows were divided into two groups of equal size based on the degree of lipid accumulation in the liver: healthy cows (0.00 fat, n = 20) and cows with fatty liver syndrome (>20% fat, n = 20). Milking was measured each day from day 7 to day 60 of lactation. Results showed that 5 to 7 days before calving, as well as 12 days after calving, BHBA concentrations were significantly higher (p
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