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Application of Protease Isolated from Bacillus sp. 158 in Enzymatic Cleansing of Contact Lenses

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Author(s): Rasika Pawar | Vasudeo Zambare | Siddhivinayak Barve | Govind Paratkar

Journal: Biotechnology
ISSN 1682-296X

Volume: 8;
Issue: 2;
Start page: 276;
Date: 2009;
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Keywords: Neutral protease | Bacillus sp. 158 | contact lenses cleansing

ABSTRACT
A neutral protease, isolated from Bacillus sp. 158 was used for removing protein deposits from contact lenses. Partial purification of the protease was carried out using ammonium sulphate and factors affecting the enzyme activity, such as assay temperature and assay pH were characterized. The optimum pH and temperature for protease were found to be pH 7.0 and 30°C, respectively. The partially purified protease was stable at temperature range of 30-40°C and pH 6-7. However, protease was maximum stable at 30°C and pH 7.0. The enzyme could be effectively used to remove protein deposit from contact lenses indicating its potential to increase in transmittance of lenses.
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