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Arbuscular Mycorrhiza on Root-Organ Cultures

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Author(s): Nishi Mathur | Anil Vyas

Journal: American Journal of Plant Physiology
ISSN 1557-4539

Volume: 2;
Issue: 2;
Start page: 122;
Date: 2007;
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Keywords: Arbuscular mycorrhiza | root-organ cultures | Glomales | in vitro | root symbioses | source of inoculum | intraradical and extraradical mycelium | mycorrhizosphere

ABSTRACT
The study of Arbuscular Mycorrhizal (AM) fungi and the AM symbiosis formed with host plant roots is complicated by the biotrophic and hypogeous nature of the mycobionts involved. To overcome this, several attempts have been made during the last three decades to obtain this symbiosis in vitro. The use of root-organ cultures has proved particularly successful. In this review, we describe the method by which root-organ cultures (transformed and nontransformed) have been obtained, together with the choice of host species, inoculation techniques and culture media. This is supported by a summary of some of the most important findings, regarding this symbiosis, that have been made at the physiological, biochemical and molecular levels.
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