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Aspects regading the capacity of gluconeogenesis regulation of the rat perfused liver (Short Notes)

Author(s): Delia Anca HAS-LAZAU

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XIII;
Start page: 53;
Date: 2006;
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Keywords: gluconeogenesis | non-glucidic precursors | CCCP | lactate-piruvate mixture | starvation conditions

The liver, also known as „the living laboratory of the organism”, is a vital organ, fulfiling a variety of functions, such as gluconeogenesis, which is glucose biosynthesis starting from non-glucidic precursors such as: lactate, faty acids, glycerol and amino acids, an extremely important biochemical process for the animal organism especially under starvation conditions, intense activity or/and pathological states (Paşca C., Kis E. 1999).By combining the liver perfusion technique and electronic microscopy techniques, we have been able to show that the liver can synthetize the necessary glucose even under starvation conditions, from the lactate-piruvate mixture that has been perfused (Mokuda and Sakamato 1997; Parrila and colab. 2003; Ross and colab. 1976; Sumida and colab. 993; Sumida and colab. 2006). We have also highlighted the CCCP(carbonil-cianid-m-clorophenylhydrazone) effect on the gluconeogenesis process, at two different final concentrations :2 μmols and 50 μmols in the Krebs-Ringer serum.The CCCP declutches the oxydative phosphorilation, making the proton gradient fade; if the concentration is low – 2 μmols – the effect lasts in time, and at high concentration – 50 μmols – it has an irreversible inhibiting effect on the gluconeogenesis.
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