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Assessing Linearity of the Parasite Varroa destructor DNA Amplification

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Author(s): ODAGIU Antonia | I. OROIAN | A. VLAIC | Al. MĂRGHITAS | P. MAZZONE | E. CAPRIO | D. DEZMIREAN

Journal: Proenvironment Promediu
ISSN 1844-6698

Volume: 2;
Issue: 4;
Start page: 226;
Date: 2009;
Original page

Keywords: Varroa | RT-PCR | linearity | efficiency

ABSTRACT
The importance of honeybee products make of disease prevention and control in honeybees one of the mainconcerns of beekeepers in the world. The PCR – RT reaction represents an alternative for amplification performed inorder to realize the Varroa destructor O. genotypization, very important stage in haoneybee resistance to parasitedescription and also in management of the treatments. The linearity data is a very important parameter and very usefulin determination of the amplification of the parasite DNA and success of the genotypization process. The amplificationefficiency was very satisfactory, fact revealed by the value of the regression line y = - 2.3103 * 26.552 together withcoefficient of determination equal (r2 = 0.9691), meaning that more than 96% of the reaction efficiency may beexplained by the process liniarity. The implementation of the RT-PCR method was successful and it represents apremise for validation process evolution.
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