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Assessment of genetic diversity among Aloe vera accessions using amplified fragment length polymorphism

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Author(s): Niraj TRIPATHI | Navinder SAINI | Sharad TIWARI

Journal: International Journal of Medicinal and Aromatic Plants
ISSN 2249-4340

Volume: 1;
Issue: 2;
Start page: 115;
Date: 2011;
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Keywords: Genetic diversity | Polymorphism | Molecular markers | AFLP

ABSTRACT
The use of herbal medicines has gain tremendously in the last decade. Amplified fragment length polymorphism (AFLP) was employed to assess the diversity in 12 elite accessions of Aloe vera collected from different locations of Madhya Pradesh, India. Among the twelve genotypes the AFLP primer combinations generated a total of 410 fragments with an average of 51.2 fragments per primer combination. In order to assess the discriminatory power of eight primer combinations used, a variety of marker attributes like unique alleles, percent polymorphism and polymorphism information content (PIC) values were calculated. Genotyping data obtained for all polymorphic fragments were used to group the accessions analyzed using the UPGMA-phenogram and principal component analysis (PCA). The cluster analysis showed a considerable level of variability among the collected genotypes of Aloe vera.
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