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ASSESSMENT OF GENETIC DIVERSITY OF REHMANNIA GLUTINOSA LIBOSCH BASED ON ISSR MARKERS

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Author(s): YANQING ZHOU, WUJUN GAO, HONGYING DUAN, FENGPING GU

Journal: Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
ISSN 1582-3571

Volume: 8;
Issue: 1;
Date: 2007;
Original page

ABSTRACT
In order to assess the genetic diversity of Rehmannia glutinosa Libosch cultivars ( lines) in Huai zone, Inter-simple sequence repeat (ISSR) was performed. Ten appropriate ISSR primers were selected from a total of 44 ISSR ones for ISSR PCR amplification. The ten primers could amplify one hundred and ten bands. Based on them, A Jaccard’s genetic similarity matrix and a dendrogram for these ten cultivars were established using SPSS 10.0 software. In this dendrogram, they could be divided into two groups : Group1 contained six individuals such as Zupei 85.5, Datian 85.5, Zupei 9302, Jinbai, Jinzhuangyuan and Datian9302; Group2 consisted of four ones such as Beijing No.1, Dahongpao, Dihuang9104 and wild dihuang. Furthermore, Principal coordinate analysis (PCA) supported the above cluster analysis; Shannon's Information index (I) is 0.3577, effective number of alleles (Ne) is 1.4037, the percentage of polymorphic loci is 71.82 % by means of POPGENE32 software; A DNA fingerprint was developed with a single primer, ISSR6, in which each of ten individuals tested had its unique fingerprint pattern and was distinguished from each other. The results revealed that ISSR method is suitable for DNA fingerprinting, identification and genetic diversity analysis of Rehmannia glutinosa in Huai zone.
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