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Augmenting transcriptome assembly by combining de novo and genome-guided tools

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Author(s): Prachi Jain | Neeraja M. Krishnan | Binay Panda

Journal: PeerJ
ISSN 2167-8359

Volume: 1;
Start page: e133;
Date: 2013;
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Keywords: De novo transcriptome assembly | Genome-guided transcriptome assembly | Transcriptome | Misassembly | Transcriptome assembly | Model assembly

ABSTRACT
Researchers interested in studying and constructing transcriptomes, especially for non-model species, face the conundrum of choosing from a number of available de novo and genome-guided assemblers. None of the popular assembly tools in use today achieve requisite sensitivity, specificity or recovery of full-length transcripts on their own. Here, we present a comprehensive comparative study of the performance of various assemblers. Additionally, we present an approach to combinatorially augment transciptome assembly by using both de novo and genome-guided tools. In our study, we obtained the best recovery and most full-length transcripts with Trinity and TopHat1-Cufflinks, respectively. The sensitivity of the assembly and isoform recovery was superior, without compromising much on the specificity, when transcripts from Trinity were augmented with those from TopHat1-Cufflinks.
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