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Avaliação in vitro da biocompatibilidade dos braquiópodos para a elaboração de um novo material

Author(s): Josu00E9 Reis dos Santos | Kristianne Santos Porta Fernandes | Manoela Domingues Martins | Sandra Kalil Bussadori | Elaine Marcu00EDlio Santos

Journal: ConScientiae Saúde
ISSN 1677-1028

Issue: 5;
Start page: 91;
Date: 2006;
Original page

The purpose of this study was to carry out an in vitro evaluation of the citotoxity of conchs, to prepare a new material with calcium carbonate or calcium phosphate. The conchs were triturates and rained in fine drops. The material were placed in glass slides and after placed in cells cultured. We used cell line NIH3T3 and gingival fibroblasts plaqued 1 x 104 cells in Petri dish. The counting was realized in optic microscopoic in one, three, five and seven days and zero, four, eight and 12 hours. After the contact of the material in cells cultured, the cells number were determined by counting the cells using Trypan blue dye exclusing assay. In both tests the material allowed cellular growing. The results conclude that: the material wasn’t citotoxity in vitro in fibroblasts cultured.
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