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Bacterial Hemoglobin gene (vgb) Transformed into Escherichia coli enhances Lead-Uptake and Minimizes it’s Adsorption

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Author(s): Khaled Khliefat | Merza H. Homady

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 3;
Issue: 9;
Start page: 1480;
Date: 2000;
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Keywords: E. coli | Lead | Pb biosorption | Pb uptake | adsorption and vgb

ABSTRACT
In Escherichia coli transformed with bacterial hemoglobin gene (vgb) has been shown to increase lead (Pb) uptake, growth yield of the cells grown in LB containing different concentration of Pb. The maximum Pb biosorption of vgb-containing and parental cells were determined to be 51 and 30.8 ppm Pb/g biomass respectively. Using growth curves by liquid and solid media the inhibitory effect og Pb on parental strain was detected at lowest concentration (10 ppm). However, in vgb-containing cells, Pb was lethal at 100ppm. The Optimum aeratin that required for the cell-containing hemoglobin gene (vgb) was lower that that of the same strain without vgb on a growth yield, Pb uptake and Pb adsorption basis. The quantity of the Pb uptake inside the cells appears to be independent on the concentration of bacterial cells. On the other hand, in E. Coli strain VHb the ability of the cells to uptake Pb was primarily a function of hemoglobin content. The higher the Pb uptake the lower the Pb adsorption. The opposite results was achieved for parental cells in which the higher Pb adsorption the lower Pb uptake.
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