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Behaviour of some apricot cultivars during in vitro multiplication phase

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Author(s): Mirela Călinescu | Maria Isac | Andi Ciobanu

Journal: Scientific Papers of the Research Institute for Fruit Growing Pitesti, Romania
ISSN 1584-2231

Volume: XXVI;
Start page: 144;
Date: 2010;
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Keywords: Explants | nutritive media | subculture | hormonal balance

ABSTRACT
This paper presents relevant data regarding behaviour of 14 apricot cultivars during in vitro multiplication phase. The biological material was represented by meristems prelevated from one year old branches. In this phase 3 growing media were used: Murashige & Skoog (1962), Fossard (1972) and Lepoivre (1977), supplemented with: dextrose (40 g/l), GA3 (0.1mg/l), BAP (1 mg/l), ANA (0.2 mg/l), and Na Fe EDTA (3.2 mg/l). The best results (in average 7.9 shoots per explants) were obtained when Fossard media was used, regardless the cultivar, the determinant role being held by the vitamin complex of this nutritive media. For all 14 cultivars the best multiplication rates was obtained whenlarge size explants of 0.3 – 0.5 mm were used.
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