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Bioassay-Guided Fractionation of the Crude Methanol Extract of Cassia singueana Leaves (0909111)

Author(s): O.J. Ode | I.U. Asuzu | I.E. Ajayi

Journal: Journal of Advanced Scientific Research
ISSN 0976-9595

Volume: 2;
Issue: 4;
Start page: 81;
Date: 2011;
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Keywords: Bioactivity | Bioassay | Column chromatography | Thin layer chromatography | Fractionation

The crude methanol extract of Cassia singueana leaves was subjected to chromatographic separation using column and thin layer chromatographic techniques. The eluted fractions were screened for bioactivity using indomethacin-induced gastric ulcer model in rats. The extract yielded eight (8) component fractions. Fraction 8 (F8) exhibited the most profound anti-ulcer activity when it completely protected rat stomachs from experimentally-induced ulcerative effects caused by 40 mg/kg of oral indomethacin treatment. Further fractionation of F8 gave two second generation fractions (A and B). Sub-fraction 8A or 8B demonstrated a comparatively reduced in vivo anti-ulcer protective ability compared to the parent fraction (F8). The bioactive effects of the subfractions against indomethacin-induced ulcerogenic tendencies in rats appeared to be synergistic.
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