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Biochemical and enzymatic alterations in mice injected with honeybee (Apis indica) venom gland extract

Author(s): Shoeb Ahmad and Ravi Kant Upadhyay*

Journal: Journal of Pharmacy Research
ISSN 0974-6943

Volume: 4;
Issue: 7;
Start page: 1965;
Date: 2011;
Original page

Keywords: Honeybee | Apis indica venom | lethality | serum enzymes | bio-molecules | LD50

In the present investigation, toxicity of honeybee venom gland extract on the activity of different biochemical and enzymatic parameters was assessed. For this purpose honeybee venom extract purified on a Sepharose CL-6B-200 gel filtration column and eluted fractions were collected in form of two protein peaks. Thecalibration curve showed the molecular weight of purified Apis indica venom proteins in a range of 14.3-63.0 kDa. Albino mice were injected with 40% and 80% of LD50 of purified venom extract and there was found a significant elevation in serum glucose (14.10%), pyruvic acid (18.41%), uric acid (18.68%), cholesterol (3.05%), total lipid (80.95%), free amino acids (37.50%), while serum protein content was inhibited 20.19% after 8 hour of treatment. A massive depletion ofstored glycogen in liver, rectus abdominus, gastrocnemius muscle, atria and ventricle was 43.03%, 35.59%, 48.98%, 54.11%, 43.02% respectively after 6 hr of venom treatment. Venom extract injected albino mice has shown increase in serum acid phosphatase (ACP) up to 63.63%, 37.50% in ALP, 61.11% in GPT and 48.27% in GPT, 25.45% in LDH whereas the level of AChE was found to be significantly reduced 31.04%. This study clearly point out the venom extract inducedstress and toxicity of Apis indica venom on biochemical and enzymatic parameters, which are important in maintaining the body physiology and necessary for life.

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