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Bioinformatics Analysis of Distribution of Microsatellite Markers (SSRs) / Single Nucleotide Polymorphism (SNPs) in Expressed Transcripts of Prosopis Juliflora: Frequency and Distribution

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Author(s): Sablok G | N.S.Shekhawat

Journal: Journal of Computer Science & Systems Biology
ISSN 0974-7230

Volume: 01;
Issue: 01;
Start page: 087;
Date: 2008;
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Keywords: Prosopis juliflora | insilico analysis | Expressed Sequence Tag (EST) | Microsatellite markers | SNP Markers

ABSTRACT
The present paper aims to identify the distribution of Microsatellite markers (SSRs)/Single Nucleotide Polymorphism(SNPs) as a resource tools for the analysis of interspecies hypervariability in Prosopis spp. Microsatellites are ubiquitously repeated extension of 1-5 bp motif extended throughout the genome. The dbEST division of Genbank contains 1467 ESTs of Prosopis which have been utilized for the development of genic microsatellitemarkers (SSRs). Locally installed assembling program was used for the cluster analysis of the EST. Analysis was performed on a Linux Cluster system. The analysis of the putative unigenes has been shown to have most abundant motif of A/T followed by dinucleotide AG/CT and trinucleotide repeat AAG/CTT. EST-derived SNPs are becoming the resources for the development of SNP markers. The relative rates of development of the high throughput computational methods for the detection of SNPs (Single Nucleotide Polymorphism) and small indels(insertion / deletion) has has gained wide applications in the field of the molecular markers.
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