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Biological and serological characterization of viruses of summer squash crops in Yugoslavia

Author(s): Dukić Nataša M. | Krstić Branka B. | Vico Ivana M. | Katis N.I. | Papavassiliou Chryssa | Berenji Janoš B.

Journal: Journal of Agricultural Sciences
ISSN 1450-8109

Volume: 47;
Issue: 2;
Start page: 149;
Date: 2002;
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Keywords: summer squash crop | plant viruses | zucchini yellow mosaic potyvirus | watermelon mosaic potyvirus 2 | cucumber mosaic cucumovirus | bioassay | serology

A survey on summer squash open field crops was carried out during 2000 and 2001 in order to identify the major viruses infecting these crops in different localities. Plants showed different types of symptoms: mild mosaic, chlorotic spotting, distinctive mosaic, blistering of leaf lamina leaf yellowing, deformation of leaf lamina, knobbed fruits and stunting of plants. The symptoms were very variable but showed the viral nature of the investigated summer squash diseases. The collected samples were tested by bioassay and by two serological methods ELISA and EBIA using cucumber mosaic cucumovirus (CMV), zucchini yellow mosaic potyvirus (ZYMV), watermelon mosaic potyvirus 2 (WMV-2), zucchini yellow flack potyvirus (ZYFV) watermelon mosaic potyvirus 1 (WMV-1), squash mosaic comovirus (SqMV) and cucurbit aphid-borne yellows polerovirus (CABYV) polyclonal antisera. In all tested samples single or mixed infection with ZYMV, CMV and WMV-2 was detected. The most prevalent virus infecting summer squash was ZYMV. This is the first report of ZYMV, the most destructive virus infecting cucurbits, in Yugoslavia. It was also proven that the identified viruses are transmissible by Aphis gossypii in a non-persistent manner, but possible role of seed in virus transmission was not confirmed.
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