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Biomarkers of Oxidative DNA Damage and Soluble Fas/Fas Ligand in Type 2 Diabetic Patients

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Author(s): Mohamed H. Mahfouz | Ibrahim A. Emara | Ghada A. Omar

Journal: American Journal of Applied Sciences
ISSN 1546-9239

Volume: 9;
Issue: 4;
Start page: 450;
Date: 2012;
Original page

Keywords: Type 2 diabetes | 8-hydroxy-2'-deoxyguanosine (8-OHdG) | apoptosis | Reactive Oxygen Species (ROS) | Malondialdehyde (MDA)

ABSTRACT
Problem statement: Involvement of oxidative stress in the pathogenesis of diabetic complications has been proposed. 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a new sensitive biomarker of the oxidative DNA damage in vivo. Apoptosis via Fas/Fas Ligand (FasL) interactions has been proposed to be a major T-cell-mediated effector mechanism in autoimmune diabetes. This study was undertaken to investigate whether the serum levels of 8-OHdG and circulating soluble Fas/Fas Ligand, two transmembrane glycoproteins involved in apoptosis, are altered in patients with type 2 diabetes. Approach: 8-OHdG, sFas and sFasL were measured with the ELISA method in twenty normal controls (group I), thirty patients with type 2 DM (duration ranged from 1-3 years, group II) and in thirty patients with type 2 DM (duration ranged from 5-10 years, group III). Also, serum glucose (Fasting and postprandial), HbA1c, insulin, lipid profile (total cholesterol, triacylglycerol, HDL-c and LDL-c) and serum malondialdehyde (MDA) level were determined. Results: The patients with a long duration of diabetes were poorly controlled and had significantly higher levels of HbA1c (p
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