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Biosynthesis of Amyloglucosidase by Aspergillus niger Using Wheat Bran as Substrate

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Author(s): Ikram-ul-Haq | H. Ashraf | S. Omar | M.A. Qadeer

Journal: Pakistan Journal of Biological Sciences
ISSN 1028-8880

Volume: 5;
Issue: 9;
Start page: 962;
Date: 2002;
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Keywords: Amyloglucosidase | aspergillus niger | acetate buffer | glucose | ammonium sulphate

ABSTRACT
The present study is concerned with the production of amyloglucosidase by Aspergillus niger GCUCM-36. Effect of addition of different carbon sources and nitrogen sources on the production of enzyme was investigated. The enzyme formation was maximum (1180 IU/g/min) in the presence of glucose (1.0%) and NH4Cl (1.5% nitrogen). The production of enzyme reached maximum, (1180 IU/g/min) at 48 hours after incubation.
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