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Biotechnological aspects concerning the ergosterol obtaining from yeasts

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Author(s): Elena MOLODOI | Agafia USATÎI | Nadejda EFREMOVA | Natalia CHISELIŢA | Ludmila FULGA

Journal: Analele Universitatii din Oradea, Fascicula Biologie
ISSN 1224-5119

Volume: TOM XX;
Issue: 1;
Start page: 12;
Date: 2013;
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Keywords: yeast | Saccharomyces carlsbergensis | sterols | ergosterol | culture medium | millimeter waves

ABSTRACT
This paper reports on the experimental results for selection of Saccharomyces carlsbergensis CNMN-Y-15 yeast strain with high potential for ergosterol biosynthesis; determination of morpho-cultural and physiologo-biochemical characteristics of this yeast strain; establishment of the effect of low-intensity millimeter waves on ergosterol biosynthesis. Research results contributed to the elaboration of technological flow for obtaining of high yield of ergosterol from yeasts. The significant increasing of ergosterol content is possible due to the utililization of optimized nutritive medium with manganese acetate (0.012 g/L) and glucose (40.0 g/L); the treatment of inoculum with low-intensity millimeter waves for 20-30 minutes.
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