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Blockade of P-selectin reduces neutrophil infiltration into the ischemia-reperfusion induced murine testis

Author(s): Celebi M. | Alberta Paul

Journal: Acta Veterinaria
ISSN 0567-8315

Volume: 59;
Issue: 5-6;
Start page: 449;
Date: 2009;
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Keywords: antibodies | neutrophils | P-selectin | testis | torsion

Germ cell apoptosis after ischemia-reperfusion (IR) of the testis is dependent on neutrophil recruitment to the testis. Intravascular adhesion molecules like the P- and E- selectins play an important role in this recruitment. The purpose of this study was to inhibit neutrophil recruitment to the IR- induced testis by using a function-blocking monoclonal anti-mouse P-selectin antibody. Adult mice were subjected to a 2 h period of testicular torsion (ischemia) followed by detorsion (reperfusion). Ten minutes after the onset of reperfusion mice received either the function-blocking monoclonal P-selectin antibody (FBMAB group) or the isotype-matched control antibody (IMCA group). Separate groups of mice underwent a sham operation (SO group) or received 500 ng of TNF╬▒ (IF group) to induce inflammation. Mice were sacrificed 24 hr after reperfusion and testiscular interstitial cells were isolated and analyzed for the presence of neutrophils by means of flow cytometry. The function-blocking monoclonal P-selectin antibody reduced neutrophil recruitment to the IR-induced testis significantly. Therefore, blocking P-selectin may be therapeutically beneficial to protect postischemic testis.
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