Academic Journals Database
Disseminating quality controlled scientific knowledge

Cabeçalho da página Sistema Eletrônico de Editoração de Revistas/Open Journal Systems Ajuda do sistema/Journal Help Usuário/User Logado como: antonioborges * Meus periódicos * Perfil * Sair do sistema Idioma Conteúdo da revista Pesquisa/Search Procurar/Browse * Por Edição/By Issue * Por Autor/By Author * Por Título/By Title * Outras revistas/Other Journals Tamanho de fonte Make font size smaller Make font size default Make font size larger Informações * Para Leitores/For Readers * Para Autores/For Authors * Para Bibliotecários/For Librarians Palavras-chave Buiatria Ciência Básica Forragicultura Morfologia Animal Nutrição Animal Nutrição animal Parasitologia Piscicultura Produção Animal Produção animal Produção de Aves Produção de Suínos Produção de aves Produção de bovinos Produção de equinos Reprodução Animal Reprodução animal Sanidade Animal Sanidade animal Tecnologia de Alimentos enzimas * Capa * Sobre * Página do usuário * Pesquisa/Search * Atual * Anteriores * Notícias Capa > v. 12, n. 1 (2011) > Barcellos EFEITO DOS MEIOS DILUÍDORES MMC (MÍNIMA CONTAMINAÇÃO) E LGM (LACTOSE-GEMA MODIFICADO) NA VIABILIDADE DO SÊMEN DE CÃES CONSERVADO POR 24 HORAS EM EQUITAINER®.

ADD TO MY LIST
 
Author(s): Bianca Perez Barcellos | Marco Antônio Rocha | Luciano Jamas | Felipe Zandonadi Brandão | Luis Garcia Nogueira | Tania Goes de Pinho

Journal: Ciência Animal Brasileira
ISSN 1518-2797

Volume: 12;
Issue: 1;
Start page: 126;
Date: 2011;
Original page

Keywords: Dogs | sperm | cooling.

ABSTRACT
The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC. No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics.
Affiliate Program     

Tango Jona
Tangokurs Rapperswil-Jona